The editors – themselves experienced RNA researchers – have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. To this end, all the techniques covered are treated with the user in mind, including detailed practical advice and an invaluable "troubleshooting" section. The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolation, characterization and functional analysis of RNA. All pertinent laboratory methods are presented in detail, including RNA synthesis, modification and ligation, Sequence and structure determination of RNA, isolation and purification of ribonucleoproteins, RNA crystallization, single molecule studies on RNA, analysis of splice variants, RNA imaging in vivo, modeling and prediction of RNA structure, RNA microarrays, Aptamer libraries and SELEX technology, RNA interference. The book is helpful for molecular biologists, biochemists and biotechnologists and of interest to every researcher experimenting with RNA. It contains 932 pages and costs 315.00 Euro.