Resin Microsopy and On-Section Immunochemistry
G.R. Newman, J.A. Hobot
Since antibody tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation.
Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilties and results are discussed so that an understanding of the techniques can be aquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application. Part I. Resin Embedding, Part II. On-Section Immunolabelling.
2001. 38 Abb. 2 Tab. XX. 276 Pages, Berlin-Heidelberg-New York-Paris-Tokyo-Hong Kong: Springer-Verlag, Euro 81,29, ISBN 3-540-67277-X
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