SEC and MALS characterize antibodies

A new application note from Wyatt Technology shows that analysis using size-exclusion chromatography (SEC) followed by multi-angle light scattering (MALS) with UV and RI detectors is a powerful tool for characterizing antibody products. The technique can determine absolute molar masses and levels of dimerization and glycosylation, helping researchers optimize the binding of engineered antibodies to their target cells or molecules. The MALS instrument used was a Wyatt Dawn Eos with Optilab DRI and HP1100 UV detectors. The Dawn Eos is the world’s most advanced commercial instrument for absolute macromolecular characterization, claims Wyatt. It is the only multi-angle light scattering (MALS) detector capable of determining radius and molar mass results simultaneously, and is able to calculate masses directly from the variation of the light scattering pattern. It works with absolute precision, meaning that there is no need to refer to standards or assumptions a priori.