Microarray slides with covalent probe binding

Slide E microarray slides from Schott Nexterion have active surface chemistries to immobilize oligonucleotides. The active layer contains epoxy groups that form an immediate and irreversible covalent bond with all the probe’s nucleophilic groups, without requiring UV cross-linking or reduction.

Amino modification is only necessary when spotting short oligonucleotides. The surface chemistry is stable and remains active even during long spotting runs, while regular and reproducible spot morphology allows the use of high-density microarrays. For spotting PCR products, Nexterion offers slides with aminosilane and aldehyde coatings. The aminosilane slides, Nexterion Slide A and Slide A+, are particularly flexible in their applications. Using the same protocol, PCR molecules and even longer oligonucleotides can be spotted on the aminosilane surface. As both slide types also work with common PCR protocols, users can change over to Schott Nexterion slides without a time-consuming re-optimization of the assay process.